PUBLICATIONS

2024

The antibacterial activity of a prophage-encoded fitness factor is neutralized by two cognate immunity proteins

Alexei, A.G.,Bullen, N.P., Garrett, S.R., Sychantha, D., Whitney, J.C.

Journal of Biological Chemistry S0021-9258(24)02509-2 (2024)

Targeting bacterial nickel transport with aspergillomarasmine A inhibits virulence-associated Ni-dependent enzymes

Sychantha, D., Chen, X., Koteva, K., Prehna, G., Wright, G.D.

Nature Communications 15: 4036 (2024)

Allosteric inhibition of tRNA synthetase Gln4 by N-pyrimidinyl-β-thiophenylacrylamides exerts highly selective antifungal activity

Puumala, E., Sychantha, D., Lach. E., Reeves, S., Nabeela, S., Fogal, M., AkshatKumar, N., Johnson, J.W., Aspuru-Guzik, A., Shapiro, R.S, Uppuluri, R., Kalyaanamoorthy, S., Magolan, J., Whitesell, L., Robbins, N., Wright, G.D., Cowen. L.E.

Cell Chemical Biology 31, (4): P760-775.E17 (2024)

Synthesis of lipid-linked precursors of the bacterial cell wall is governed by a feedback control mechanism in Pseudomonas aeruginosa

Marmot, L.S., Orta, A.K, Baileeves, B.W.,  Sychantha, D., Fernández-Galliano, A., Li, Y.E., Greene, N.G., Corey, R.A., Stansfeld, P.J., Clemons, W.M., Bernhardt, T.G.

Nature Microbiology 9: 763–775 (2024)

2023

Identification of a broadly conserved family of enzymes that hydrolyze (p)ppApp

Ahmad, S., Gordon, I.J., Tsang, K.K., Alexei, A., Sychantha, D., Colautti, J., Trilesky, S., Kim, Y., Wang, B., Whitney, J.C.

Proceedings of the National Academy of Sciences 120: (14) e2213771120 (2023)

2022

An ADP-ribosyltransferase toxin kills bacterial cells by modifying structured non-coding RNAs

Bullen, N.P., Sychantha, D., Thang, S.S., Culviner, P.H., Rudzite, M., Ahmad, S., Shah, V. S., Filloux, A., Prehna, G., Whitney, J.C.

Molecular Cell 82: 1-15 (2022)

A widespread family of gene clusters produces ClpP inhibitors

Culp, E., Sychantha, D., Hobson, C., Pawlowski, A.J., Prenha, G., Wright, G.D.

Nature Microbiology 7: 451-462 (2022)

Three-dimensional structure and optimization of the metallo-β-lactamase inhibitor aspergillomarasmine A

Koteva, K.*, Sychantha, D.*, Rotondo, C.M.*, Hobson, C., Britten, J., Wright, G.D.

ACS Omega 7: 4170-4184 (2022)

2021

Aspergillomarasmine A inhibits metallo-β-lactamases by selectively sequestering Zn2+

Sychantha, D., Rotondo, C.M., Terhani K.H.M.E, Martin, N.I., Wright, G.D.

Journal of Biological Chemistry 297: (2) 100918 (2021)

2020

Structural basis for the O-Acetyltransferase function of the extracytoplasmic domain of OatA from Staphylococcus aureus

Jones C.J., Sychantha, D., Howell, P.L., Clarke A.J.

Journal of Biological Chemistry 295: (24) 8204-13 (2020)

Evolution-guided discovery of antibiotics that target peptidoglycan remodelling

Culp E., Waglechner, N., Wang, W., Fiebig-Comyn, A., Yen-pang, H., Koteva, K., Sychantha, D., Coombes, B., VanNieuwnhze, M., Brun, Y., Wright, G.D.

Nature 578: 582-587 (2020)

Suppression of β-lactam resistance by aspergillomarasmine A is influenced by the class of the β-lactam antibiotic partner

Rotondo, C.M*., Sychantha D*., Koteva, K., Wright G.D.

Antimicrobial Agents and Chemotherapy 64 (4) e01386-19 (2020)

Peptidoglycomics: Examining compositional changes in peptidoglycan between biofilm- and planktonic-derived Pseudomonas aeruginosa

Anderson E.M., Sychantha D., Brewer, D., Clarke, A.J. Geddes-McAlister J., Khursigara C.M.

Journal of Biological Chemistry 295 (2): 504-51 (2020)

2019

Assays for the enzymes catalyzing the O-acetylation of bacterial cell wall polysaccharides

Brott A.S., Sychantha D., Clarke A.J.

Methods in Molecular Biology 1954: 115-136 (2019)

2018

Mechanistic pathways for peptidoglycan O-acetylation and de-O-acetylation.

Sychantha, D., Brott, A.S, Jones, C.J., Clarke, A.J.

Frontiers in Microbiology 9: 2332 (2018)

Peptidoglycan modification by the catalytic domain of Streptococcus pneumoniae OatA follows a ping-pong bi-bi mechanism of action

Sychantha, D., and Clarke, A. J.

Biochemistry 57 (16): 2394-2401 (2018)

Molecular basis of the attachment of S-layer proteins to the cell wall of Bacillus anthracis

Sychantha, D., Chapman, R.N., Bamford, N.C., Boons, G.J., Howell, P.L., Clarke, A.J.

Biochemistry 57(13): 1949-1953 (2018)

PatB1 is an O-acetyltransferase that decorates secondary cell wall polysaccharides

Sychantha, D., Little, D.J., Chapman, R.N., Boons, G.J., Robinson, H., Howell, P.L., Clarke, A.J.

Nature Chemical Biology 14: 79-85 (2018)

2014 - 2017

In vitro characterization of the antivirulence target of Gram-positive pathogens, peptidoglycan O-acetyltransferase A (OatA)

Sychantha, D., Jones, C., Little, D.J., Moynihan, P. J., Robinson, H., Galley, N.F., Roper, D.I., Dowson, C.G, Howell, P.L., Clarke, A.J.

PLOS pathogens 13(10): e1006667 (2017)

O-Acetylation of the serine rich repeat glycoprotein GspB is coordinated with accessory Sec transport

Seepersaud, R., Sychantha, D., Bensing, B.A., Clarke, A.J., Sullam, P.M.

PLOS Pathogens 13(8): e1006558 (2017)

Accumulation of peptidoglycan O-acetylation leads to altered cell wall biochemistry and negatively impacts pathogenesis factors of Campylobacter jejuni

Ha, R., Frirdich, E., Sychantha, D., Biboy, J., Taveirne, M.E., Di Rita, V.J., Vollmer, W., Clarke, A.J., Gaynor, E.C.

Journal of Biological Chemistry 291: 22686 - 22702. (2016)

Chemical biology of peptidoglycan O-acetylation and deacetylation

Moynihan, P.J., Sychantha, D., Clarke, A.J.

Bioorganic Chemistry 54: 44-50.2 (2014)